Journal: Cell Death & Disease

Article Title: Downregulation of Engulfment and cell motility 1 (Elmo1) induces quiescence and resistance to poly(I:C)-induced apoptosis in endothelial cells

doi: 10.1038/s41419-025-08341-1

Figure Lengend Snippet: a Transcript levels of selected genes associated with apoptosis before (0 h) and after (24 h) the poly(I:C) treatment. Transcript levels of each gene are shown as TPM (transcript per million). Data are mean ± SE ( N = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, n.s . not significant. b Representative images of Mock, siNT and siELMO1 cells 24 h after the poly(I:C) stimulation. Cells were treated with or without 20 ng/ml of caspase-8 inhibitor Z-IETD-FMK throughout the experiment. Bar = 20 μm. c Cell death in (b) was quantified as in Fig. . Data are mean ± SD ( N = 3). *** p < 0.001. d Western blot of full-length and cleaved caspase-8, -3 and -7 before (0 h) and after (4 h, 8 h) the stimulation with poly(I:C). Beta-Actin was detected as a loading control. Images are representative of three independent experiments.

Article Snippet: The absence of mycoplasma contamination was confirmed using the Mycoplasma PCR Detection Kit (Abcam, Cambridge, MA, USA), Caspase-8 inhibitor Z‐IETD‐FMK was purchased from R&D systems (Minneapolis, MN, USA).

Techniques: Western Blot, Control

Journal: Cell Death Discovery

Article Title: Alamandine alleviates hypertension and renal damage via oxidative-stress attenuation in Dahl rats

doi: 10.1038/s41420-022-00822-y

Figure Lengend Snippet: a Ala (1.0 and 10 µg/mL) inhibited the increases of KIM-1 and NGAL in HK-2 cells induced by NaCl. b Ala (1.0 µg/mL) inhibited the increases in cleaved ( c )-caspase3, c-caspase8, and c-poly(ADP-ribose) polymerase (PARP) in HK-2 cells induced by NaCl. The data are expressed as mean ± standard error of the mean (SEM); n = 8 ( a ) or 4 ( b ) in each group. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Article Snippet: After electrophoretic separation and transfer of bands to a polyvinyl difluoride (PVDF) membrane, the proteins on the membrane were probed with antibodies against c-caspase3 (#9661; Cell Signaling Technology), c-caspase8 (#9496; Cell Signaling Technology), c-PRAP (#5625; Cell Signaling Technology), Rac1 (#4651; Cell Signaling Technology), p22 phox (ab80896; Abcam, Shanghai, China), p47 phox (ab181090; Abcam), NOX4 (ab133303; Abcam), and PKC (#2056; Cell Signaling Technology).

Techniques:

Journal: Cell Death Discovery

Article Title: Alamandine alleviates hypertension and renal damage via oxidative-stress attenuation in Dahl rats

doi: 10.1038/s41420-022-00822-y

Figure Lengend Snippet: a Ala inhibited the increase of PKC in the HK-2 cells induced by NaCl. b PKC overexpression reversed the attenuating effects of Ala on the increases of cleaved (c)-caspase3, c-caspase8, and c-poly(ADP-ribose) polymerase (PARP) induced by NaCl in HK-2 cells. The data are expressed as mean ± standard error of the mean (SEM); n = 4 in each group. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Article Snippet: After electrophoretic separation and transfer of bands to a polyvinyl difluoride (PVDF) membrane, the proteins on the membrane were probed with antibodies against c-caspase3 (#9661; Cell Signaling Technology), c-caspase8 (#9496; Cell Signaling Technology), c-PRAP (#5625; Cell Signaling Technology), Rac1 (#4651; Cell Signaling Technology), p22 phox (ab80896; Abcam, Shanghai, China), p47 phox (ab181090; Abcam), NOX4 (ab133303; Abcam), and PKC (#2056; Cell Signaling Technology).

Techniques: Over Expression